How do I calculate peptide reconstitution?

Divide the peptide amount (mg) by the BAC water volume (mL) to get concentration. Then divide your desired dose by the concentration to get the injection volume. Multiply by 100 for U-100 syringe units.

What is the difference between MCG and MG?

1 mg (milligram) = 1,000 mcg (micrograms). Most peptides are dosed in mcg because the amounts are very small.

How much BAC water should I add to a peptide vial?

2-3 mL is standard for most peptides. This gives a manageable concentration for accurate syringe measurement.

PepsCalc — Peptide Calculator

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1

Peptide in Vial

How many milligrams (mg) of peptide powder is in your vial? Check the label.

mg

This is the total amount of peptide powder in your vial before mixing. It's printed on the vial label — common sizes are 2mg, 5mg, 10mg, or 15mg.

2

Bacteriostatic Water

How much BAC water will you add (or already added) to the vial?

mL

Bacteriostatic water (BAC water) is sterile water with 0.9% benzyl alcohol that you add to dissolve the peptide powder. 2–3 mL is standard for most peptides. More water = easier to measure small doses but more liquid to inject.

3

Your Dose

How much peptide do you want per injection?

mcg

01000

mcg = micrograms — one millionth of a gram. Most peptides are dosed in mcg.
mg = milligrams — one thousandth of a gram. 1 mg = 1,000 mcg.
IU = International Units — used for HCG, HGH. Conversion varies by peptide.

DRAW TO

0 units

on your U-100 insulin syringe

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Concentration (mg/mL)

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Doses per vial

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Your Dose

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Vial Size

📊 How many doses in this vial? ▾

💧 Best water amount? ▾

Most peptides work best with 2–3 mL of BAC water. This keeps the concentration manageable so you're not pulling tiny fractions of a syringe unit.

Too little water (1 mL) = very concentrated, hard to measure small doses accurately.
Too much water (5+ mL) = large injection volumes, may need multiple syringes.

🔬 MCG vs MG explained ▾

1 mg (milligram) = 1,000 mcg (micrograms)
1 mcg (microgram) = 0.001 mg

Most peptides are dosed in mcg because the amounts are tiny. For example, BPC-157 at 250 mcg = 0.25 mg.

IU (International Units) are used for HCG and growth hormone. The conversion depends on the specific peptide.

📚 Learn

🧪 What is Reconstitution?

Reconstitution is the process of mixing a powdered peptide with sterile water so it can be injected. Here's how:

Step 1: Remove caps from the peptide vial and BAC water vial
Step 2: Draw the desired amount of BAC water into a syringe
Step 3: Slowly inject the water into the peptide vial along the glass wall (don't squirt directly onto the powder)
Step 4: Gently swirl — never shake. Let it sit until fully dissolved (clear liquid)

📐 MCG vs MG vs IU

Unit	Full Name	Equals
mg	milligram	1,000 mcg
mcg (μg)	microgram	0.001 mg
IU	International Unit	varies by peptide
mL	milliliter	volume of liquid

Example: 250 mcg of BPC-157 = 0.25 mg. If your vial has 5 mg total, that's 20 doses of 250 mcg.

💉 Syringe Types

U-100 (most common) — 100 units per mL. Each small line = 1 unit = 0.01 mL. This is the standard insulin syringe used for peptides.

U-50 — 50 units per 0.5 mL. Smaller syringe, same unit spacing. Good for very small doses.

U-40 — 40 units per mL. Mainly for veterinary use. Do NOT use with U-100 calculations.

🧊 Storage & Handling

Before mixing: Store powder in fridge or freezer. Room temp is OK short-term.
After mixing: Always refrigerate (2–8°C / 36–46°F). Use within 30 days.
BAC water: Room temperature is fine. Lasts 28 days after first puncture.
Never freeze reconstituted peptides — ice crystals destroy the structure.
Avoid light — store in the box or wrapped in foil.

🧪

PepsCalc

Free peptide reconstitution calculator
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⚠️ For educational and research purposes only. This is not medical advice. Always consult a qualified healthcare professional before using any peptide. Verify all calculations against your vial label.